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Frogs had been housed in synthetic polycarbonate tanks both in solitary and environments that are mixed-sex

Frogs had been housed in synthetic polycarbonate tanks both in solitary and environments that are mixed-sex

Pets and husbandry

An overall total of 27 male and 19 feminine DGFs had been found in this research. Pets had been housed at Mississippi State University’s (MSU) Amphibian Conservation Lab (Starkville, MS, United States Of America) through the duration of the research. All pets were captive reared in the Memphis Zoo and Omaha’s Henry Doorly Zoo, and had been used in MSU before the start of research. Animal many years ranged from roughly 4 to 6 yrs . old through the scholarly research duration. Your pet husbandry methods and therapy protocols had been authorized by Mississippi State University’s Institutional Animal Care and make use of Committee (IACUC #10–082).

Frogs had been housed in plastic polycarbonate tanks (46 Ч 66 x 30 cm; Habitat Systems Limited, Diverses Moines, Iowa, United States Of America) in both single and mixed-sex environments with between one and four conspecifics. Animals had been formerly implanted with passive transponder that is integrated (PIT tags) making it possible for consistent recognition of an individual through the entire research. About 50 % regarding the tank had been covered with moistened organic moss, and a little synthetic hide (Medium Reptile Hide; LLL Reptile and provide Co, Oceanside, CA) ended up being supplied as extra address when it comes to frogs. Frogs had access to aged regular water in bowls and had been maintained a sun light period (Starkville, MS, 33.4625° N, 88.8200° W) throughout the analysis.

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